The need for sustainable aquaculture has promoted research into the use of probiotics on aquatic organisms. The initial interest was focused on their use as growth promoters and to improve the health of animals; however, new areas have been found, such as their effect on reproduction or stress tolerance, although this requires a more scientific development.

The growth of aquaculture as an industry has accelerated over the past decades; this has resulted in environmental damages and low productivity of various crops. The need for increased disease resistance, growth of aquatic organisms, and feed efficiency has brought about the use of probiotics in aquaculture practices. The first application of probiotics occurred in 1986, to test their ability to increase growth of hydrobionts (organisms that live in water). Later, probiotics were used to improve water quality and control of bacterial infections. Nowadays, there is documented evidence that probiotics can improve the digestibility of nutrients, increase tolerance to stress, and encourage reproduction.

Enzymes are proteins that assist chemical reactions in the body. Although livestock's gut microflora produce some enzymes, they do not all the enzymes in sufficient quantities to hydrolyze NSP, proteins or phytates to enable absorption of all nutrients. Enzyme supplementation improves the quality of feedstuffs and livestock, resulting in higher profit margins. Enzyme addition helps to produce more meat per animal at a much lower cost while improving the overall long-term health of the herd. Vet costs and mortality rates also decline with the use of enzyme supplements in animal feed. Enzymes come from a variety of natural sources, including plant extracts, animals, and microorganisms. Enzymes are widely used by the agricultural, brewing, biofuel, dairy, and rubber industries to boost production and minimize costs.

Bacillus amyloliquefaciens is very common probiotic, which has been proven beneficial to the breeding industry. In recent years, the application in aquaculture has attracted widespread attention.